Scientific stuff
Barbara's skills
Molecular biology
- Standard molecular biology and DNA manipulation techniques for cloning and heterologous protein expression and production in Escherichia coli: gene expression vector construction, PCR, RT-PCR
- High quality total RNA and mRNA isolation; genomic DNA extraction
- Sequencing of genomic DNA
- Strong knowledge of the antibody phage display technology: panning and selection of scFv from a library of antibodies, affinity maturation of a library of antibodies, antibody characterization (BIAcore, sequencing, gel filtration, affinity column)
Cell biology and biochemistry
- Immunologic techniques: ELISA, immunoprecipitation, western blot, dot blot, 2D-SDS polyacrylamide gel, immunofluorescence, ADCC, CDC, B-cell depletion, Apoptosis analysis, migration assays, Flow cytometry
- Protein and antibodies purification
- Strong experience in handling of insect and mammalian (mice, rat, human, hamster) cells and in vivo systems (chicken embryo)
- Different transfection and transduction methods
- Lentivirus-based viral particle production (working experience in biosafety level 2)
Other skills and informations
- Languages: Italian (mother tongue), English, (oral and written), German (oral and written), French (school knowledge)
- Informatics: excellent knowledge of Windows and Mac based platforms and related software: MS Office, Acrobat, Photoshop, EndNote, DNA Strider, Plasmap, MacDraw, EnzymeX, ImageJ
Barbara's publications
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B. Guarino
HIV-Derived Lentiviral Vectors Engineered for Regulated Transgene ExpressionPhD Thesis, ETHZ 2005 [pdf]
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B. Mitta, M. Rimann, M. Ehrbar, V. Djonov, M. Ehrengruber,
J. Kelm, M. Fussenegger (2002)
Advanced modular self-inactivating lentiviral vectors for multigene interventions in mammalian cells and in vivo transductionNucleic Acids Res, 30(21):e113 [pdf]
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B. Mitta, C. Weber, M. Rimann, M. Fussenegger (2004)
Characterization of Self-Inactivating Human and Non-Human Lentiviral Expression Vectors Engineered For Streptogramin-Adjustable Transgene ExpressionNucleic Acids Res, 32(12):e106 [pdf]
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B. Mitta, C. Weber, M. Fussenegger (2004)
In-Vivo Transduction of HIV-1-Derived Lentiviral Particles Engineered For Macrolide-Adjustable Transgene ExpressionJournal of Gene Medicine [pdf]
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B. Mitta, M. Rimann, M. Fussenegger (2004)
Detailed Design and Comparative Analysis of Protocols for Optimized Production of High-Performance HIV-1-derived Lentiviral ParticlesMetabolic Engineering [pdf]
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F. Viti, P. Pedrioli, B. Mitta, D. Neri (2001)
Phage display libraries as a source of tumour-targeting agentsChimia, 55, 206-211.
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C. Fux, B. Mitta, B. Kramer, M. Fussenegger (2004)
PDual-regulated expression of C/EBP-alpha and BMP-2 enables differential differentiation of C2C12 cells into adipocytes and osteoblastsNucleic Acids Res, 32(1):e1
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C. Diaz Sanchez Bustamante, J. Kelm, B. Mitta, M. Fussenegger (2001)
Heterologous Protein Production Capacity of Mammalian Cells Cultivated as Monolayers and MicrotissuesBiotechnology and Bioengineering
Andrea's skills
Scientific knowledge
- Classical and quantum optics, quantum electronics, electro-optics, nonlinear optics, wave propagation in anisotropic crystals, integrated optics, optoelectronics
- Laser physics, quantum physics, solid state physics, thermodynamics
- Fundamentals of electronics, structural engineering, chemistry, nuclear engineering, nuclear instrumentation
Lab experience
- Fabrication of waveguides: ion implantation, ferroelectric thin films by crystal ion slicing and bonding
- Structuring: clean Room class 10000, photolithography, RIE etching, sputtering, bonding, fs laser micromachining
- Testing: interferometry, ellipsometry, waveguide coupling, waveguide mode measurements, electro-optics measurement, second-harmonic measurement, AFM surface analysis
Work experience
- Development of high power single emitter laser diodes (GaAs based). Wavelengths: 793-808-830-880-9xx nm
- Physical characterization electro-optical perfomance of laser diodes (LabView software)
- Reliability testing of laser diodes (Hardware maintenance and development, data analysis)
- Planning and realization of prototypes and pre-production samples (Front End and Back End)
- Database development for large quantity data analysis, database development for prototype production trackflow and planning
Computer Skills
- OS: Windows, OSX, Linux
- Programming: fundamentals of Java, C++, HTML, SQL, Ruby
- Scientific: MATLAB (advanced), Mathematica, LabView, Femlab (Comsol Multiphysics), C2V (integrated optics)
- Other: Office, Flash, Illustrator, Photoshop, GIMP, Inkscape. MySQL, MS Access
Andrea's publications
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A. Guarino
Electro-optic microring resonators in inorganic crystals for photonic applicationsPhD Thesis 17029, ETHZ 2007. [pdf]
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A. Guarino, G. Poberaj, D. Rezzonico, R. Degl'Innocenti and P. Gunter,
Electro-optically tunable micro-ring resonators in LiNbO3Nature Photonics, 1, 407-410 (2007)[pdf]
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A. Guarino, M. Jazbinsek, C. Herzog, R. Degl'Innocenti and P. Gunter,
Optical waveguides in Sn2P2S6 by low fluence MeV He+ ion implantationOptics Express 14(6): 2344-2358, 2006 [pdf]
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A. Guarino and P. Gunter,
Nondestructive method for the characterization of ion-implanted waveguidesOptics Letters 30(18): 2412-2414, 2005 [pdf]
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D. Rezzonico, A. Guarino, C. Herzog, M. Jazbinsek and P. Gunter,
High-finesse laterally coupled organic-inorganic hybrid polymer microring resonators for VLSI photonicsIEEE Photonics Technology Letters, 18(5-8): 865-867, 2006.
- L. Mutter, A. Guarino, M. Jazbinsek, P. Gunter, M. Zgonik, and M. Doebeli
Ion implanted optical waveguides in nonlinear optical organic crystalOptics Express, 15(2) 629-638, 2007
- R. Degl'Innocenti, A. Guarino, S. Reidt, G. Poberaj and P. Gunter
Second harmonic generation of continuous wave ultraviolet light and production of β-BaB2O4 optical waveguidesApplied Physics Letters 89 (4): 041103, 2006.
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D. Haertle,A. Guarino,J. Hajfler, G. Montemezzani and P. Gunter
Refractive indices of Sn2P2S6 at visible and infrared wavelengthsOptics Express (6): 2047-2057, 2005.
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R. Piazza, A. Guarino
Soret effect in interacting micellar solutionsPhysical Review Letters, 88 (20): 208302, 2002
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Degl'Innocenti R., Reidt S., Guarino A., Rezzonico D., Poberaj G. and Gunter P.
Micromachining of ridge optical waveguides on top of He+-implanted β-BaB2O4 crystals by femtosecond laser ablationJournal of Applied Physics, 100, 113121, 2006.
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Herzog Ch, Aravazhi S. , Guarino A., Schneider A., Poberaj G. and Gunter P.
Epitaxial K1-xNaxTa0.66Nb0.34O3 thin films for optical waveguiding applicationsJournal of the Optical Society of America B
